Submit or Track your Manuscript LOG-IN

Comparative Transcriptome Analysis of the Ovary and Testis in Noble Scallop (Chlamys nobilis)

Comparative Transcriptome Analysis of the Ovary and Testis in Noble Scallop (Chlamys nobilis)

Fan Sigang1, Guo Yihui1* and Xu Youhou2

1Key Laboratory of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, PR China
2Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Beibu Gulf University, Qinzhou, PR China

*      Corresponding author: guoyihui@scsfri.ac.cn

ABSTRACT

Noble scallop (Chlamys nobilis) is an economically important cultured marine bivalve shellfish common in southern China. Investigation on the molecular regulatory mechanisms of gonadal maturation in scallop is critical in the aquacultural industry. Here, gonads in maturing stage were obtained from noble scallops and sequenced using an Illumina high-throughput sequencer, producing 6.68 and 6.70 Gb of data for the ovary and testis, respectively. Reproduction-related genes, including vasa, nanos, and vitellogenin, and sex-determining genes, such as FoxL2, Dmrt, and sox9, were detected. Transcriptome comparison revealed 2,842 differentially expressed genes (DEGs), of which 591 exhibited biased expression in the ovary and 2,251 exhibited biased expression in the testis. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses of the DEGs were conducted. Results showed that GO terms and KEGG pathways related to protein glycosylation, fatty acid biosynthetic processes, hydrolase activity, and AMPK were enriched in the ovary, whereas those related to male organ formation and spermiogenesis were enriched in the testis. The glycosphingolipid biosynthesis pathway was identified for the first time in a mollusc testis.The present study provides the first transcriptomic analysis of C. nobilis, which will help clarify the molecular mechanisms of gonadal maturation.
 

To share on other social networks, click on P-share. What are these?

Pakistan Journal of Zoology

February

Vol. 53, Iss. 1, Pages 1-400

Featuring

Click here for more

Subscribe Today

Receive free updates on new articles, opportunities and benefits


Subscribe Unsubscribe