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Comparative Analysis of Immunological and Genomic Outcomes of Dengue Virus Outbreak in Pakistan

Comparative Analysis of Immunological and Genomic Outcomes of Dengue Virus Outbreak in Pakistan

Muhammad Shahid1, Iram Amin1, Samia Afzal1,*, Zareen Fatima1 and Muhammad Idrees2

1Division of Molecular Virology, National Center of Excellence in Molecular Biology, Punjab University, 87-West Canal Bank Road, Thokar Niaz Baig, Lahore
2Hazara University, GT Road Dodial, Mansehra

Muhammad Shahid and Iram Amin have contributed equally to this article.

*     Corresponding author: samiaraza@live.com

ABSTRACT

The mortality and morbidity due to dengue fever is increasing worldwide at a frightful rate. Emergence and re-emergence of dengue fever in previous epidemics has been documented globally, including Pakistan. We conducted a comprehensive study and compared the immunological and genomic status of dengue outbreak in Pakistan through ELISA and PCR. In this study 720 samples were collected from different diagnostic centre’s/laboratories of Pakistan for ELISA and PCR tests. Out of 703 dengue positive samples, 93.5% were anti-dengue IgM positive, 71.5% were anti-dengue IgG positive and 81.3% were anti-dengue NS1 positive. Among these seropositive samples, 39.66%, 40.15% and 36.88% were PCR positives, respectively. This immunological and genomic study reveals that there were more seropositive patients than RNA positive ones. Serotype-2 remained the main causative serotype of the outbreak of dengue viral infection, started in the commencement of year 2013 and continues to infect Pakistani population till now.
 

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Pakistan Journal of Zoology

October

Vol. 51, Iss. 5, Pages 1599-1997

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