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Chicken Embryo Fibroblasts are Effective Cells to Study the Function of Chicken Neurexophilin 1 Gene

Chicken Embryo Fibroblasts are Effective Cells to Study the Function of Chicken Neurexophilin 1 Gene

Dongmei Sun, Meiling Chen, Hafiz Ishfaq Ahmad, Yun Liu, Xunping Jiang and Guiqiong Liu*

Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People’s Republic of China

*      Corresponding author: liuguiqiong@mail.hzau.edu.cn

 

 

ABSTRACT

Neurexophilin has been proved to be a differentially expressed gene in chicken sperm storage tubules. In this work, Immunocytochemistry, immunofluorescence and Western-blot were performed to detect the expression of neurexophilin 1 in epithelial cells of chicken utero-vaginal junction, chicken embryo fibroblasts (DF-1 cells) and Hela cells. The immunocytochemistry results showed that neurexophilin 1 was expressed in both the epithelial cells of chicken genital tract and DF-1 cells. Immunofluorescence revealed that neurexophilin 1 was expressed in cytoplasm as well as cell nucleus of chicken fibroblasts and Hela cells. Western-blot analysis displayed neurexophilin 1 in the cell and cell culture fluid of DF-1 cells and Hela cells, and higher expression of neurexophilin 1 in cell culture fluid of DF-1 cells than that of Hela cells. These findings suggested that DF-1 cells are effective and a better choice than Hela cells to do researches on the function of neurexophilin 1 gene.
 

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Pakistan Journal of Zoology

August

Vol. 50, Iss. 4, Pages 1199-1600

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