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Characterization of Thermophilic Bacteria Anoxybacillus rupiensis and Cultivation in Agroindustrial Wastes Isolated from Hot Spring in Chakwal, Pakistan

Characterization of Thermophilic Bacteria Anoxybacillus rupiensis and Cultivation in Agroindustrial Wastes Isolated from Hot Spring in Chakwal, Pakistan

Faiza Jabeen1,*, Bushra Muneer2 and Javed Iqbal Qazi3

1Department of Zoology, Government College University, Faisalabad
2Institute of Industrial Biotechnology, Government College University, Lahore
3Department of Zoology, University of the Punjab, Lahore

*      Corresponding author: faizajabeen38@yahoo.com

 

Fig. 1.

Phylogenetic tree of the isolates which have the highest homology with Anoxybacillus. Consensus neighbour-joining phylogenetic unrooted tree from sequences of the 16S rRNA gene, encompassing Anoxybacillus species.

Fig. 2.

Effect of different pH on growth and extracellular protein produced by Anoxybacillus rupiensis JF83 (A) and JF82 (B) following 24 h of incubation at 60°C and 140 rpm.

Fig. 3.

Effect of different temperatures on growth and extracellular protein produced by Anoxybacillus rupiensis JF83 (A) and JF82 (B) following 24 h at optimized pH and 140 rpm.

Fig. 4.

Effect of different nitrogen sources on growth and extracellular protein production produced by Anoxybacillus rupiensis JF83 (A) and JF82 (B) at optimized conditions.

Fig. 5.

Cultivation of Anoxybacillus rupiensis JF83 (A) and JF82 (B) in 2% sugarcane bagasse without nutrients (SCB) and sugarcane bagasse containing nutrients (SCB-N) for 24 and 48 h at initial pH 6.

Fig. 6.

Cultivation of Anoxybacillus rupiensis JF83 (A) and JF82 (B) in 2% whey without nutrients and whey with nutrients (whey-N) (A), whey containing all the ingredients of FJ medium except chitin (whey-N) (B) for 24 and 48 h at initial pH 6.

Fig. 7.

Cultivation of Anoxybacillus rupiensis JF83 (A) and JF82 (B) in 2% SCB and whey nutrients (SCB+whey-N) (A) and SCB and whey containing all the ingredients of FJ medium except chitin (SCB+whey-N) (B) for 24 and 48 h at initial pH 6.

Fig. 8.

Cultivation of Anoxybacillus rupiensis JF83 (A) and JF82 (B) in 2% wheat straw and wheat straw containing nutrients (wheat straw-N) for 24 and 48 h at initial pH 6.

Pakistan Journal of Zoology

December

Vol. 51, Iss. 6, Pages 1999-2399

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