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Characterization of Staphylococcus aureus from Sore Throat Patients: Association Among Host Immune Evasion and Toxin Genes

Characterization of Staphylococcus aureus from Sore Throat Patients: Association Among Host Immune Evasion and Toxin Genes

Arifa Mehreen1, Iram Liaqat2,Muhammad Arshad3, Muzzamil Waheed4 and Najma Arshad1,*

1Department of Zoology, University of the Punjab, Lahore 
2Department of Zoology, Govt. College University, Lahore
3Department of Zoology, University of Education, Lahore
4Department of Pharmacy, Government College University, Faisalabad

Arifa Mehreen and Iram Liaqat contributed equally to this work.

*      Corresponding author: najmaarshad@gmail.com; najmaarshad.zool@pu.edu.pk

 

Fig. 1.

Phylogenetic tree of 16s rRNA gene sequence of MRSA-42, showing genetic relatedness of different strains with each other.

Fig. 2.

Electrophoretic profile of spa polymorphism product amplified through polymerase chain reaction (PCR). Lane L, molecular marker of 1Kb; Lanes 1, 2 and 3, isolates with 1100bp and 1300bp spa gene product and spa negative, respectively.

Fig. 3.

Electrophoresis profile in 2% agarose gel showing PCR amplification products for the S. aureus enterotoxins L., DNA ladder, 1-3 are sed, seb and sea.

Fig. 4.

Electrophoresis profile in 2% agarose gel showing multiplex PCR amplification products for the S. aureus exo-foliative toxin tst toxin mec A and fem A genes. Lane L, DNA marker (100-bp ladder); Lanes 2 to 7, PCR amplicon; Lane 1 and 8, negative control; Lane 2, eta, etb, tst, mecA, and femA; Lane 3, mecA plus femA; Lane 4, tst and femA; Lane 5, eta and fem A; Lane 6, etb and femA; Lane 7, eta and femA.

Fig. 5.

Frequency of host immune evasion and mec A (A) and toxin genes (B) in S. aureus isolated from sore throat patients in Punjab.

Fig. 6.

Association among Coa and spa genes.

Pakistan Journal of Zoology

December

Vol. 50, Iss. 6, Pages 1999-2398

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