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Characterization of CLCN5 and Expression Profile under Low-Salinity Stress in Takifugu rubripes

Characterization of CLCN5 and Expression Profile under Low-Salinity Stress in Takifugu rubripes

Lin Ye, Jie-Lan Jiang*, Jia Xu, Rui-Ting Liu, Yin-Qiu Tian, Qing-Qing Zang, Jia-Yi Cao and Ming-Guang Mao*

Key Laboratory of Mariculture and Stock Enhancement in North China’s Sea, Ministry of Agriculture and Rural Affairs, Key Laboratory of Fish Applied Biology and Aquaculture in North China, Liaoning Province, College of Fisheries and Life Sciences, Dalian Ocean University, Dalian 116023, China

 
* Corresponding author: jielanjiang@dlou.edu.cn; mmg@dlou.edu.cn

ABSTRACT

Voltage-gated chloride channel 5 (CLCN5) transports hydrogen ions into the nucleolus and removes chloride ions, which helps the cell to maintain its pH value and osmotic pressure. To reveal the role of CLCN5 under low-salinity stress, a cDNA sequence of CLCN5 (TrCLCN5) was cloned from Takifugu rubripes using the reverse transcription PCR (RT-PCR) technique. The results showed that TrCLCN5 cDNA contained a complete open reading frame of 2505 bp continuously encoding 834 amino acid residues. Sequence alignments revealed that the TrCLCN5 cDNA is highly homologous (96.2% and 96.1%) with that of Takifugu bimaculatus and Takifugu flavidus. SignalP and TMHMM analysis showed that TrCLCN5-deduced protein was a type III transmembrane protein and lacked a typical signal peptide. Conserved Domain analysis revealed that there was a Voltage_CLC domain and two CBS domains located in the TrCLCN5 deduced protein. qPCR analysis showed that TrCLCN5 was highly expressed in the intestine, kidney and liver, and up-regulated in gills under low-salinity stress in 3h to 6h, which indicates that TrCLCN5 may play an important role in the responses to acute low-salinity stress and would be useful in further understanding low-salinity adaption in T. rubripes.

 

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Pakistan Journal of Zoology

June

Vol. 54, Iss. 3, Pages 1001-1500

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