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Characteristics of Structure and Nucleotide Polymorphism of the American Mink (Neovison vison) Growth Hormone Gene

Characteristics of Structure and Nucleotide Polymorphism of the American Mink (Neovison vison) Growth Hormone Gene

Jakub Jan Skorupski

Department of Ecology and Environmental Protection, University of Szczecin, Wąska 13 St., 71-415 Szczecin, Poland
Department of Genetics and Animal Breeding, West Pomerania University of Technology, Piastów 45 Ave., 70-311 Szczecin, Poland

*      Corresponding author: jakub@gajanet.pl

ABSTRACT

The nucleotide sequence, variation and gene-structure of the American mink (Neovison vison) growth hormone gene was analyzed. For this purpose set of 3 amplicons were sequenced, including 5’-UTR and all exons and introns. The study involved 389 animals. The sequencing results revealed the presence of 5 exons (10 bp, 161 bp, 117 bp, 162 bp, 201 bp) and 4 introns (245 bp, 171 bp, 176 bp, 290 bp), with a total length of 1745 bp. Fourteen polymorphic variable sites were identified: 12 SNP substitutions, one single nucleotide deletion and one ins/del polymorphism. The assessment of informativeness and conclusiveness of the identified variable sitesshows that the highest ability to differentiate the American mink growth hormone gene genotypes has tri-allelic polymorphism g.1219C>G (PIC = 0.3039), and three di-allelic polymorphisms - g.616G>C, g.846A>G and g.931C>T (PIC>0.2400). The recognized SNPs enrich the database of single nucleotide polymorphisms for American mink.
 

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Pakistan Journal of Zoology (Associated Journals)

June

Vol. 49, Iss. 3, Pages 761-1149

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