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Buserelin Acetate Reduces Mortality and DNA Defragmentation of Bovine Sperm Cells Exposed to Oxidative Stress

Buserelin Acetate Reduces Mortality and DNA Defragmentation of Bovine Sperm Cells Exposed to Oxidative Stress

Alicja Kowalczyk1, Marian Kuczaj2, Anna Szul3 and Ewa Czerniawska-Piątkowska4*

1Wrocław University Of Environmental and Life Sciences, Department of Environment, Animal Hygiene and Welfare, Chełmońskiego 38C, Wrocław 
2Wrocław University Of Environmental and Life Sciences, Institute of Animal Breeding, Chełmońskiego 38C, Wrocław
3Malopolska Biotechnic Centre Ltd, Krasne 32
4Department of Animal Reproduction Biotechnology and Environmental Hygiene West Pomeranian University of Technology in Szczecin,ul. Klemensa Janickiego 29, 71-270 Szczecin, Poland

*      Corresponding author: ewa.czerniawska-piatkowska@zut.edu.pl

Fig. 1.

Flow cytometry-obtained mean values of the viability from frozen-thawed bovine semen samples in the presence and the absence of buserelin acetate. Values are mean ± S.D. of thawed bovine spermatozoa in all the concentration of buserelin acetate. Values in the same row with different letters mean significantly different (P < 0.05). (n = 10).

Fig. 2.

CASA - obtained mean values of the percentage of motile sperm and progressive motility from frozen-thawed bovine semen samples in the presence and the absence of buserelin acetate. Values are mean ± S.D. of thawed bovine spermatozoa in all the concentration of buserelin acetate. Values in the same row with different letters mean significantly different (P < 0.05). (n = 10).

Fig. 3.

Flow cytometry - obtained mean values of the DNA integrity from frozen-thawed bovine semen samples in the presence and the absence of buserelin acetate. Values are mean ± S.D. of thawed bovine spermatozoa in all the concentration of buserelin acetate. Values in the same row with different letters mean significantly different (P < 0.05). (n = 10).

Pakistan Journal of Zoology

August

Vol. 52, Iss. 4, Pages 1225-1630

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