Study was conducted on breeding and mortality of Royal Bengal Tiger (Panthera tigris tigris Linnaeus 1758). There are five big cat species in the genus Panthera and tiger is the biggest one. Data were collected by recording observations on breeding of Royal Bengal Tiger in captivity at Bahawalpur Zoo from June 2012 to July 2014 (two years) on prescribe sheets. Data from June, 2003 to December, 2011 (nine years) were collected from zoo management. In two years study Tiger breed once and produce four cubs (female) while in nine years it breed three times; first three cubs (one male and two female), two and one cubs were produced in 2003, 2005 and 2006, respectively. Six days estrous cycle and 107 days gestation period was observed in tigress. Mortality of one cub of 32 days in August, 2013 and second cub in Jannaury, 2014 of 173 days was observed due to congenital abnormality of liver. Parent tigress (Rani) was also died in May, 2014 due to respiratory failure and cardiac arrest and female cub died in 2005 due to canabalism.