Development of Simple Sequence Repeats (SSR) by Transcriptome in Chinese Mitten Crab (Eriocheir sinensis H. Milne Edwards)
Development of Simple Sequence Repeats (SSR) by Transcriptome in Chinese Mitten Crab (Eriocheir sinensis H. Milne Edwards)
Fukuan Du1,2, Yan Li2, Zhixin Wen1, Ruguo Chen1 and Pao Xu2*
ABSTRACT
The Chinese mitten crab (Eriocheir sinensis) is one of the most studied and deconomically important crustaceans in China. However, the production scale does not meet the market demand. This resulted in excessive fishing, which almost caused extinction of the species in the the Yangtze River. Therefore, it is very urgent to carry out genetic breeding to improve yield. In this study, we developed SSR markers by transcriptome, which could be used to genetic research. In this study, we performed transcriptome sequencing of six libraries from hepatopancreas samples in E. sinensis. After removing low-quality reads, short reads and reads belonging to mitochondria, a total of 278,260,960 clean reads corresponding to mRNAs were obtained, these reads covered a total of 34,780,598,273 bases. A total of 48,657 SSR loci were recognized, of which 10,310 unigene sequences contain more than one SSR. These genetic markers are ideal molecular markers for the following investigating genetic diversity, constructing genetic maps, and marker assisted selection.
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