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Bioinformatics analysis of polymerase and envelope genes of hepatitis B virus in Egypt

Bioinformatics analysis of polymerase and envelope genes of hepatitis B virus in Egypt

Amal Mahmoud1&2 and Medhat H. Hashem3

1Bioinformatics Dep., Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Egypt
2Alkhwarizmi Center for Bioinformatics, Egypt
3Animal Biotechnology Dep., Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Egypt

ABSTRACT

Hepatitis B virus (HBV) genotype C is a leading risk factor for hepatocellular carcinoma (HCC). Eight HBV isolates from Sohag governorate, Egypt were confirmed by polymerase chain reaction (PCR), resulted in 1110 nt correspond to partial sequence of polymerase (pol) and envelop (env) genesTwo HBV isolates were associated with HCC (Sohag1 and 7). Phylogenetic analysis of the nucleotide sequence and the amino acid of the pol or env genes revealed that 7 out of the 8 isolates were closely related to genotype D. Only Sohag 1-HCC isolate was clustered with genotype C which is known as risk factor of HCC. Multiple sequence alignment resulted in unique amino acid substitutions (V320→L and N464→T) and (I284→L, T300→I and K334→R) in pol and env genes respectively. Further prospective studies are needed to confirm the role of these mutations in the development of HCC.

 

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Journal of Virological Sciences

July

Vol. 8, Pages 1-27

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