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Bioethanol Production from Saw Dust through Simultaneous Saccharification and Fermentation

Bioethanol Production from Saw Dust through Simultaneous Saccharification and Fermentation

Ammara Afzal1, Tazmeen Fatima1, Maham Tabassum1, Muhammad Nadeem2*, Muhammad Irfan3, Quratulain Syed2

1Center for Earth and Environmental Studies, University of the Punjab New Campus, Lahore, Pakistan.
2Food and Biotechnology research center (FBRC), Pakistan Council of Scientific & Industrial Research (PCSIR) Laboratories Complex Ferozpur Road, Lahore, Pakistan.
3Department of Biotechnology, University of Sargodha, Sargodha, Pakistan.

*Corresponding author: Muhammad Nadeem

mnadeempk@yahoo.com

 

ABSTRACT

This study was designed to compare the efficient production of ethanol in separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) process. Fungal strains of Saccharomyces cervisae, Trichoderma viride, Trichoderma koninji and Trichoderma harzianum was used for subsequent process using saw dust as substrate. Three major processes convert lignocelluloses to bioethanol i.e. pretreatment of biomass, enzymatic hydrolysis of raw material and fermentation. The sawdust was pretreated with 2.5% NaOH and further processed for SHF and SSF. Maximum saccharification was observed by T. viride (10.17%) followed by T. harzianum (9.19%) and T. koninji (6.91%) at 35˚C for 48h. Among both strategies, SSF combination of T. viride and S. cerevisiae gave maximum ethanol production (3.88%) after 12 days of fermentation at 30˚C.
 

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Punjab University Journal of Zoology

June

Vol. 34, Iss. 1, pp. 1-104

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