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Bio-management of root knot nematode and root rot disease by antagonistic fungi and rhizobacteria

A. K. Chaubey and Satyandra Kumar

Nematology Laboratory, Department of Zoology, C.C.S. University, Meerut- 250 005, E-mail:akc.nema@gmail.com

ABSTRACT

Effects of four fungi and six rhizobacteria isolates from the rhizosphere of vegetable crops was studied for suppression of root knot nematode, Meloidogyne incognita and wilt fungus, Fusarium oxysporum f. sp. lycopersici on tomato in laboratory and pot culture experiment. All the isolates were isolated by serial dilution and cultured in liquid protein supplemented broth medium and culture filtrates (CF) were prepared. In in-vitro test single eggmasses of uniform size were kept in 5ml CF in 5cm diameter Petri plates. Antifungal activity was tested by dual culture of wilt fungus with antagonist fungus and bacteria isolates in PDA media plates. In in-vivo test soil was amended with antagonist and wilt fungal and bacterial isolates 2 x 106 CFU/ml and 2 x 108 CFU/ml respectively as talc formulation @ 0.2% (w/w). Twenty one days old tomato seedlings were transplanted after dip treatment in antagonist fungal and bacterial CF in earthen pots filled with amended soil. Seven days after transplanting, the seedlings were inoculated with second stage juveniles of M. incognita @ 2J2/g soil. The exposure of M. incognita eggs and juveniles to the culture filtrates showed high inhibition in egg hatching and caused high mortality of juveniles than the control (P< 0.05). The microorganisms which caused high nematicidal activity also showed antifungal activity in dual plate culture test in vitro. All the antagonists showed reduction in root knot and root rot disease and promote plants growth as the length and weight of root and shoot in pot trials on tomato plants. Keywords: Antagonistic fungi, root knot nematode, wilt fungus, bio-management
 

 

 

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The Journal of Plant Protection Sciences

December

Vol. 7, Iss. 1-2, Pages 1-18

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