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Bio-Methane Production Potential Assays of Organic Waste by Anaerobic Digestion and Co-Digestion

Bio-Methane Production Potential Assays of Organic Waste by Anaerobic Digestion and Co-Digestion

Nafeesa Batool1*, Javed Iqbal Qazi1, Nida Aziz1, Ali Hussain2 and Syed Zawwar Hussain Shah3

1Department of Zoology, University of the Punjab, Lahore, Pakistan
2Department of Wildlife and Ecology, University of Veterinary and Animal Sciences, Lahore, Pakistan
3Department of Physics, Riphah International University, Islamabad, Pakistan

*      Corresponding author:



The present study was conducted to investigate biochemical methane production potential (BMP) of the most abundant feed-stocks i.e., dairy manure (DM) and food wastes (FW) in agrarian countries by anaerobic digestion (AD) and co-digestion (Co-AD) under mesophilic (35 ± 2 ﹎) settings at substrate to microorganism (S / M) ratio of 1.0 based on volatile solids (VS) for 30 days. Solids [Total solids (TS), volatile solids (VS), fixed solids (FS) and their ratios as (VS / TS)] determinations were also carried out for process monitoring. The highest cumulative biogas yield of 459.4 mL /g VS was observed in the co-digestion of DM + FW with 58.63 and 41.36 % CH4 and CO2 contents. BMP of the microcrystalline cellulose (MCC), FW and DM were observed as 401.2, 389.61 and 358.7 mL /g VS with CH4 and CO2 contents of 54.1 and 35.9, 51.13 and 42.2, 56.66 and 40 %, respectively. The volatile solids reductions were 82, 79, 59 and 71 % for MCC, FW, DM and FW + DM, respectively. These results indicate that FW and DM are very desirable substrates for AD and their Co-AD upturns the biogas generation efficiency. Thus, we can conclude that BMP assays are extremely useful to determine the amount of bio-methane and bio-degradability of the organic substrates and would facilitate in the preliminary selection systems for the field scale applications especially in framework of developing countries.

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Pakistan Journal of Zoology


Vol. 52, Iss. 3, Pages 825-1224


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