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Baohuoside I Inhibits Benzo(a)Pyrene-Induced Metastasis in A549 Lung Cancer Cells by Modulating STAT3 and EMT

Baohuoside I Inhibits Benzo(a)Pyrene-Induced Metastasis in A549 Lung Cancer Cells by Modulating STAT3 and EMT

Muhammad Khan1,*, Amara Maryam2, Hafiz Abdullah Shakir1, Javed Iqbal Qazi1 and Yongming Li3,*

1Department of Zoology, University of the Punjab, Quaid-i-Azam Campus, Lahore 54590, Pakistan
2College of Basic Medical Sciences, Dalian Medical University, Dalian, Liaoning116044, P.R. China
3Institute of Traditional Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, P.R. China

*      Corresponding authors: khan_zoologist@ymail.com; liyongming1979@sina.com

 

Fig. 1.

Effect of BHS-1 on proliferation and cell death of A549 and A549/B cells. A, Chemical structure of BHS-1; B, A549 and A549/B cells were treated with indicated concentrations of BHS-1 for 24 h and cell proliferation was determined by MTT assay. Data are expressed as Mean±SEM of 3 different experiments. Columns not sharing the same superscript letter differ significantly (P<0.05); C, A549 and A549/B cells were treated with 30 μM BHS-1 for 24 h and cell death was evaluated by live/dead assay; D, Data from live/dead assay are expressed as Mean±SEM of 3 different experiments. Columns not sharing the same superscript letter differ significantly (P<0.05).

Fig. 2.

Effect of BHS-1 on BaP-induced cell migration: A, determination of migration ability of cells by wound healing assay as described in Materials and Methods section; B, determination of migration ability of cells by Transwell chamber assay as described in Materials and Methods section; C, statisctical analysis of data from Transwell chamber assay. Data from live/dead assay are expressed as Mean±SEM of 3 different experiments. Columns not sharing the same superscript letter differ significantly (P<0.05).

Fig. 3.

Western blot analysis of STAT3 activation: A, A549 and A549/B cells were treated with DMSO and BHS-1 (15μM) for 24 h and expression and phosphorylation of STAT3 was determined by Western blot analysis; B, protein expressions of p-STAT3/STAT3 from 3 different experiments. Columns not sharing the same superscript letter differ significantly (P<0.05).

Fig. 4.

Modulatory effect of BHS-1 on expression of Cox-2, MMP-2 and -9 in response to chronic exposure of BaP: A, A549 and A549/B cells were treated with DMSO and BHS-1 (15μM) for 24 h and expressions of Cox-2, MMP-2 and -9 were determined by Western blot analysis; B, protein expressions from 3 different experiments were quantified by densitometry analysis. Similar superscript letters having * differ significantly with similar superscript letters without * at P<0.05.

Fig. 5.

Modulatory effect of BHS-1 on BaP-induced EMT: A, A549 and A549/B cells were treated with DMSO and BHS-1 (15μM) for 24 h and expressions of N-cadherin and E-cadherin was determined by Western blot analysis; B, protein expressions from 3 different experiments were quantified by densitometry analysis. Similar superscript letters having * differ significantly with similar superscript letters without * at P<0.05.

Cell viability (%)

Pakistan Journal of Zoology

December

Vol. 50, Iss. 6, Pages 1999-2398

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