Bacterial Expression and Purification of Antigenic Recombinant Protein Encoded by Hepatitis Delta Virus Antigen of Pakistani Isolate
Iram Amin1,2*, Shazia Rafique1, Nadeem Ahmed1, Muhammad Shahid1, Samia Afzal1, Tahir Rehman Samiullah1, Mohsin Ahmed Khan1 and Muhammad Idrees1,3
PCR amplification of HDV gene fragment, Lane 1-9, HDV DNA positive of 405bp and Lane 10 showing negative control, Lane 11, DNA marker of 50bp.
Restriction digestion analysis of clone in expression vector: Lane A, DNA marker of size 100bp; Lane B, first band digested of peT28a vector of size 5369bp and second band of HDAg-An gene fragment of size 402bp. Lane C, undigested clone; Lane D, ladder 1Kb.
A, SDS-PAGE of purified protein showing expression of 14KDa protein fragment. B, Western Blot representing 14KDa protein fragment of HDAg-An purified protein.