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Pakistan J. Zool., Vol. 50, Iss. 1, pp 175-181

Pakistan J. Zool., Vol. 50, Iss. 1, pp 175-181

Muhammad Saqlain1, Humaira Kalsoom1, Muhammad Fiaz1,2, Abid Mahmood1, Rizwan Aziz Qazi4 ,Waseem Safdar5, Pakeeza Arzoo Shaiq1,Bernard M.Y. Cheung3 and Ghazala Kaukab Raja1,*

1Department of Biochemistry, PMAS Arid Agriculture University, Rawalpindi, Pakistan
2Department of Pathology, Shaheed Zulfiqar Ali Bhutto Medical University, Islamabad, Pakistan
3Department of Medicine, The University of Hong Kong, Queen Mary Hospital, Hong Kong
4Department of Medicine, Shaheed Zulfiqar Ali Bhutto Medical University, Islamabad, Pakistan
5State Key Laboratory of Food Science and Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, People’s Republic of China

*      Corresponding author: ghazala@uaar.edu.pk

Fig. 1.

PCR-RFLP based genotyping of FABP2 Ala54Thr polymorphism. The Hha1 restriction enzyme digested PCR products were run in well 1-9 and 10-15. The M lane represents the 100bp DNA ladder run along with samples for product size estimation. According to results, as shown on gel, the un-cut 180bp PCR products of samples 1-3, 6, 9 and 11-15 represent 54Thr homozygous genotype of FABP2 gene. The completely cut 5 and 10 number samples represent Ala54 homozygous genotypes while samples 4, 7, and 8 with both un-cut (180bp) and cut (99 and 81bp) PCR products clearly indicate the heterozygous Ala54Thr genotypes of FABP2 gene.

Pakistan Journal of Zoology

December

Vol. 49, Iss. 6, Pages 1937-2341

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