Submit or Track your Manuscript LOG-IN

Association of ChREBP Expression and Polymorphism with Serum Biochemical Indices in Cherry Valley Duck

Association of ChREBP Expression and Polymorphism with Serum Biochemical Indices in Cherry Valley Duck

Hua-Lun Luo, Yi-Yu Zhang*, Yuan-Yu Qin and Lei Wu

Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, College of Animal Science, Guizhou University, South Campus, Huaxi District, Guiyang, Guizhou 550025, P.R. China

*      Corresponding author: zyy8yyc@163.com

 

ABSTRACT

ChREBP (carbohydrate-responsive element-binding protein) plays a crucial role as a central regulator of lipid synthesis and glycolysis in animal liver. In this study, the relative quantitative real-time PCR analysis indicated that the duck ChREBP mRNA is widely expressed in all examined tissues. ChREBP mRNA level was the highest in abdominal fat and the lowest in gizzard. The g.247075G>A silent mutation in exon 10 was first identified by direct sequencing approach, and resulted in three genotypes of AA, GA and GG. Association analysis demonstrated that the liver ChREBP mRNA expression level was significantly positive or negative effect on serum total protein (TP), albumin (Alb), triglyceride (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) (P<0.05 or P<0.01). The g.247075G>A mutation was significantly association with these measured serum biochemical indexes except for LDL-C. Significant additive effects were detected for all detected serum biochemical indexes except for LDL-C between allele A and G, and significant dominance effects for TP. These findings suggested that ChREBP might be a crucial candidate genes for the selection of duck lipid content.
 

To share on other social networks, click on P-share. What are these?

Pakistan Journal of Zoology

August

Vol. 51, Iss. 4, Pages 1203-1598

Featuring

Click here for more

Subscribe Today

Receive free updates on new articles, opportunities and benefits


Subscribe Unsubscribe