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Antibacterial Efficacy of Manuka Honey against New Delhi Metallo-β-Lactamase Producing Gram Negative Bacteria Isolated from Blood Cultures

Antibacterial Efficacy of Manuka Honey against New Delhi Metallo-β-Lactamase Producing Gram Negative Bacteria Isolated from Blood Cultures

Muhammad Usman Qamar1, 2, Sidrah Saleem1, Usman Arshad1, Muhammad Farhan Rasheed3, Hasan Ejaz4, Naveed Shahzad5 and Shah Jahan6,*

1Department of Microbiology, University of Health Sciences, Lahore, Pakistan
2Department of Microbiology, Faculty of Life Sciences, Government College University, Faisalabad, Pakistan
3Department of Pathology, Allama Iqbal Medical College, Lahore, Pakistan
4Department of Pathology, The Children’s Hospital and The Institute of Child Health, Lahore, Pakistan
5School of Biological Sciences, University of the Punjab, Lahore, Pakistan
6Department of Immunology, University of Health Sciences, Lahore, Pakistan

*      Corresponding author: shahjahan@uhs.edu.pk

Fig 1

Modified Hodge’s test for phenotypic carabpenemase detection. T, test organism; P, positive control (K. pneumoniae); N, negative control (E. coli).

Fig 2

Double disc potentiation method for the phenotypic detection of MBL producing bacteria. Positive results are shown by enlarged zone of inhibtion. MEM, meropenem disc; ETA, ertapenem disc; EDTA, ethylene-diamine tetra acetic acid.

Fig 3

PCR product of blaNDM-1gene on 1.5% agarose gel. N, negative control; P, positive control; T1-T5, test sample; L, DNA ladder.

Fig 4

Agar well diffusion assay for the determination of antibacterial activity of manuka honey. White arrows indicate the zone of inhibition (mm) produced by undiluted manuka honey against NDM-1 producing bacteria.

Pakistan Journal of Zoology (Associated Journals)

October

Vol. 49, Iss. 5, Pages 1547-1936

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