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Assessment of Mitochondrial ATPase 8/6 Genes Based Genetic Diversity in Sindh Ibex (Capra aegagrus Blythi)

Assessment of Mitochondrial ATPase 8/6 Genes Based Genetic Diversity in Sindh Ibex (Capra aegagrus Blythi)

Javeria Zafar1, Asif Nadeem1*, Maryam Javed1, Fehmeeda Fatima1, Wasim Shehzad1, Ghulam Abbas1, Rajput Zahid Iqbal2 and Muhammad Muddassir Ali1

1Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences Lahore
2Shaheed Benazir Bhutto University of Veterinary and Animal Sciences, Sakrand

JZ and AN made equal contributions to this article.

*      Corresponding author: asifnadeem@uvas.edu.pk

 

ABSTRACT

The current study was designed to characterize mitochondrial ATPase8/6 genes in Sindh ibex and to determin its phylogenetic relationship with other capra species. Blood samples were collected from Kirthar National Park, Sindh, Pakistan. PCR product was sequenced bi-directionally using dideoxy chain termination method after mitochondrial ATPase8/6 genes amplification. Polymorphism, Genetic diversity and Phylogenetic analysis were carried out by the MUSCLE, DnaSP and MEGA6 tools. Total of 20 variations at different positions were found in aligned sequence results. Sequence conservation was observed for the ibex population. The overall results showed a close evolutionary relationship among Capra aegagrus, Capra nubiana, Capra falconeri, Capra hircus and Capra caucasica, demonstrating that they all were evolved from same ancestor. 

 

 

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Pakistan Journal of Zoology

February

Vol. 52, Iss. 1, Pages 1-424

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