Submit or Track your Manuscript LOG-IN

An Optional Low-Cost Method of Extracting Environmental DNA of Macro-Organisms from Filter Membranes in Large Scale eDNA Surveys

An Optional Low-Cost Method of Extracting Environmental DNA of Macro-Organisms from Filter Membranes in Large Scale eDNA Surveys

Zhi Chen1, Toshifumi Minamoto2, Longshan Lin3 and Tianxiang Gao4,*

1College of Fisheries and Life Science, Hainan Tropical Ocean University, Sanya 572022, China
2Graduate School of Human Development and Environment, Kobe University, Kobe 657-8501, Japan
3Marine Life and Ecology Laboratory, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China
4Fisheries College, Zhejiang Ocean University, Zhoushan 316022, China

*      Corresponding author: gaotianxiang0611@163.com

Fig. 1.

Water sampling sites of this study. From left to right, the map was gradually enlarged.

Fig. 2.

Sediment concentration of all water samples.

Fig. 3.

The DNA yield (copies/300 ml) of Sepiella japonica extracted from aquaculture water (indoors water sample). There was no difference in eDNA yield between the DNeasy Blood and Tissue Kit and AxyPrep DNA Gel Extraction Kit groups (p=0.061).

Fig. 4.

Agarose gel electrophoresis results of 4 field water samples. M, DNA marker.

Pakistan Journal of Zoology

February

Vol. 53, Iss. 1, Pages 1-400

Featuring

Click here for more

Subscribe Today

Receive free updates on new articles, opportunities and benefits


Subscribe Unsubscribe