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An Antimicrobial Cerebroside from the Liposoluble Constituent of Cervus Nippon Antler Velvet Layer

An Antimicrobial Cerebroside from the Liposoluble Constituent of Cervus Nippon Antler Velvet Layer

Nina Bao1,*, Yongguang Yin2 and Panpan Wang3

1School of Biological and Food Engineering, Suzhou University, Suzhou 234000, China
2School of Biological and Agricultural Engineering, Jilin University, Changchun 130022, China
3School of Foreign Studies, Suzhou University, Suzhou 234000, China

*      Corresponding author: ninabao1984@163.com

 

ABSTRACT

Using petroleum ether to extract an antimicrobial cerebroside from the liposoluble constituent of cervus nippon antler velvet layer was studied in this paper. Single-factor experiment was used to research the effect of liquid-to-solid ratio, standing time and centrifugation time on the extraction of crude cerebroside. The optimal extraction conditions for the cerebroside as follows: liquid-to-solid ratio 15, standing time 45 min, and centrifugation time 6 min. The extracted cerebroside was purified and identified by using silica gel column and TLC, respectively. IR analysis showed that the extraction has hydroxyl and amide groups, which was proved to be cerebroside. Meanwhile, bioactivity showed that the extraction has antibacterial activity, and the purified one is better than the crude one.
 

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Pakistan Journal of Zoology

October

Vol. 50, Iss. 5, Pages 1601-1998

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