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Acute Effect of Epidermal Growth Factor on Phosphate Diffusion across Intestinal Mucosa of Hens using the Ussing Chamber System

Acute Effect of Epidermal Growth Factor on Phosphate Diffusion across Intestinal Mucosa of Hens using the Ussing Chamber System

Xiaopeng Tang1,2, Rejun Fang3,4,*, Guochang Pan1,2 and Kangning Xiong1,2,*

1School of Karst Science, Guizhou Normal University, Guiyang, China
2State Key Laboratory Cultivation for Karst Mountain Ecology Environment of Guizhou Province, Guiyang, China
3College of Animal Science and Technology, Hunan Agricultural University, Changsha, China
4Hunan Co-Innovation Center of Animal Production Safety, Changsha, China

*      Corresponding author: fangrj63@126.com; xiongkn@163.com

 

ABSTRACT

The aim of this study was to investigate the effect of epidermal growth factor (EGF) on phosphate absorption across intestinal mucosa of hens using the Ussing chamber system. First, four Loman laying hens with similar weight (1.25±0.13 kg) were sacrificed to detect the lactate dehydrogenase (LDH) activity of isolated small intestine at time on 0 min, 30 min, 60 min, 90 min and 120 min, to provide a appropriate operation time for Ussing chamber test. Then, a total of 20 Loman laying hens with similar weight (1.25±0.13 kg) were divided into 1 of 5 treatments: i) control group (0 ng/mL EGF), ii) 50 ng/mL EGF group, iii) 100 ng/mL EGF group, iv) 150 ng/mL EGF group, and v) 200 ng/mL EGF group for using chamber test. The results showed that (i) the LDH activity of isolated intestine increased (P < 0.05) sharply during 30 min, at 60 min, the LDH activity of isolated intestines reached a high level; (ii) EGF increased (P < 0.05) the transepithelial electrical resistance (TEER, Ω.cm2) significantly; (iii) EGF inhibited (P < 0.05) the phosphate diffusion across intestinal mucosa significantly. According to the results, the Ussing chamber system can use to evaluate phosphate diffusion across intestinal mucosa, and EGF may inhibit the absorption of phosphorus.
 

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Pakistan Journal of Zoology

October

Vol. 51, Iss. 5, Pages 1599-1997

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