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API-ZYM Enzymatic Profile of Shigella dysenteriae IM Isolated from Drinking Water

API-ZYM Enzymatic Profile of Shigella dysenteriae IM Isolated from Drinking Water

Abdul Nabi Jatt1,*, Sarfraz Ali Tunio1, Shaista Bano Memon1, Abdul Sattar Qureshi2 and Muhammad Aqeel Bhutto

1Institute of Microbiology, University of Sindh, Jamshoro 76080, Sindh, Pakistan
2Institute of Biotechnology and Genetic Engineering, University of Sindh, Jamshoro 76080, Sindh, Pakistan

*      Corresponding author: abdul.nabi@usindh.edu.pk

 

ABSTRACT

The enzymatic potential of bacterial species is an essential feature to carry out a wide range of biological and physiological processes, particularly involved in the hydrolysis of complex organic materials. Several pathogenic microbial species utilize enzymes as virulence factors to cause diseases. The aim of the present study was to evaluate enzymatic activities of the bacterial strain Shigella dysenteriae IM using API-ZYM test system. Out of nineteen different enzymes, S. dysenteriae IM strain was capable to poroduce nine enzymes, i.e., alkaline-phosphatase, acidic-phosphatase, lipase, valine arylamidase, cystine arylamidase, napthol-AS-BI-phosphohydrolase, β-galactosidase, leucine arylamidase, and trypsin. While, esterase, esterase lipase, α-chymotrypsine, α-galactosidase, β-glucuronidase, α-glucosidase, β-glucosidase, N-acetyl- β-glucosaminidase, α-mannosidase, and α-fucosidase were recorded as negative. Moreover, S. dysenteriae IM showed elevated alkaline-phosphatase, leucine arylamidase, trypsin, and acidic-phosphatase activities. The present study reveals that, the API-ZYM test is the convenient, reliable and inclusive alternative to recent biological methods. This method could be applied for the identification of various enzymes on routine basis directly in bacterial cultures and biological fluids.
 

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Pakistan Journal of Zoology

June

Vol. 50, Iss. 3, Pages 799-1198

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