Molecular Detection and Pathological Investigation of Contagious Bovine Pleuropneumonia in Selected Districts of Punjab, Pakistan
Molecular Detection and Pathological Investigation of Contagious Bovine Pleuropneumonia in Selected Districts of Punjab, Pakistan
Ahsan Anjum1, Asim Aslam1, Raheela Akhtar1, Tahir Yaqub2, Muti-ur-Rehman Khan1, Rizwana Sultan3, Saba Usman1, Aneela Zameer Durrani4 and Muhammad Usman4*
ABSTRACT
Contagious Bovine Pleuropneumonia (CBPP) is an infectious respiratory disease of cattle, characterized by anorexia, fever, dyspnea, polypnea, cough, and nasal discharges and caused by Mycoplasma mycoides subsp. mycoides. This study was carried out in abattoirs of Lahore, Kasur and Jhang districts of Punjab, Pakistan. Different tissue samples were collected from 560 cattle showing respiratory signs at slaughter. Lung samples were used for detection of CBPP through PCR targeting 16S ribosomal RNA. Furthermore, lungs, liver, kidney and lymph node were collected for histopathological examination. Of the 560 samples, 49 (8.25%) were found positive for CBPP. Maximum pathological lesions (50-75% of surface area) were seen in 34.69 percent lung samples, chronic type pleurisy in 97.96 percent, maximum aggregate pathological lesion score in 65.31%, while 48.98% lung samples had 6-10 numbers of sequestra. Besides, infiltration of inflammatory cells, congestion, hemorrhages, necrosis and other degenerative changes were observed in tissue samples of infected cattle. Significantly higher (P<0.05) micro-pathological lesion scores were given to lungs, kidney and lymph node samples from infected cattle as compared to those of non-infected ones. In conclusion, CBPP, being a major factor affecting cattle production and associated economics, warrants the implementation of control measures to mitigate the economic losses associated with the disease.
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April 2020
Vol. 52, Iss. 2, Pages 425-824
