Have we found an Optimal Insertion Site in a Newcastle Disease Virus Vector to Express a Foreign Gene for Vaccine and Gene Therapy Purposes?
Have we found an Optimal Insertion Site in a Newcastle Disease Virus Vector to Express a Foreign Gene for Vaccine and Gene Therapy Purposes?
Qingzhong Yu
ABSTRACT
Different approaches have been used to investigate the most suitable insertion site for optimal expression of a foreign gene without compromising the replication of the Newcastle disease virus. Recently, inserting a green fluorescent protein (GFP) gene upstream from the GE sequences of the viral genes and subsequent quantitative measurements of the GFP fluorescence intensity, we have concluded that the P and M junction region is the optimal insertion site for a high level of foreign gene expression by a NDV vector.
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April 2015
Vol. 2, Iss. 2 Pages 15-27
