Efficient in Vitro Plant Regeneration Through Somatic Embryogenesis from Callus Induction Method for Brassica carinata
Efficient in Vitro Plant Regeneration Through Somatic Embryogenesis from Callus Induction Method for Brassica carinata
Rizwan Ullah Shah* and Iqbal Munir
ABSTRACT
A study was conducted for optimizing basal medium through somatic embryogenesis (cotyledon and hypocotyl) for callus induction followed by shoot and root regeneration of Brassica carinata. Seeds were sterilized and germinated, of which one week cotyledon and hypocotyl were used for callus formation and shoot regeneration at five different level of 1-naphthaleneacetic acid NAA (0.05, 0.1, 0.5, 1.0 and 1.5 mg/l) and 6-benzyl amino purine BAP (0.1, 0.5, 0.7, 1.0 and 1.5 mg/l), and root regeneration at four levels of indole butyric acid IBA (0.05, 0.1, 0.2 and 0.5 mg/l). Results showed that at NAA 0.05 mg/l + BAP 0.07 mg/l supplements in MS medium significantly effective in callus induction and days to callus in cotyledon and for both cotyledon and hypocotyl were observed. For shoot regeneration efficiency and days to shoots, the optimum phytohormone were observed at NAA 0.1 mg/l + BAP 1.0 mg/l along with AgNO3 supplements. The optimum regeneration efficiency of root and days to root initiation was observed with 0.2 mg/l supplement. As cotyldeon and hypocotyl slightly varied in response to these phytohormones, but overall callus induction along with shoot and root regeneration both were equally susceptible to the present experiment applied phytohormones
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