Antimicrobial Activity from Cellular, Subcellular and Molecular Fractions of Cultured Microbes against Each Other
Antimicrobial Activity from Cellular, Subcellular and Molecular Fractions of Cultured Microbes against Each Other
Mirza Imran Shahzad1,*, Anna Iqbal2, Farrah Ali1, Nuzhat Sial3, Muhammad Ashfaq4, Abul Hasanat5 and Azra Khanum6
ABSTRACT
Mankind is using antimicrobial agents in various forms since last 2000 years. The present study was designed to evaluate the antimicrobial activities of different culture microbes against each other. A total six bacteria including Salmonella typhi, Escherichia coli, Pasteuralla multocida, Lactobacillus bulgaricus, Micrococcus luteus and Staphylococcus aureius were used against each other to evaluate their antimicrobial activities. All of bacteria were cultured in LB media and further the culture of each bacterium was divided into three parts, the first part was centrifuged in order to collect supernatant. While the second and third parts were also centrifuged but however supernatants were discarded to get their pellets, which were dissolved in normal saline. Of this one part was taken for thaw and freeze treatment, while the second for ultrasonication treatment, before conducting antimicrobial activities. Zones of inhibition were measured by using disc diffusion method. Supernatant fraction of E. coli was positive against Sal. typhi and supernatant fraction of P. multocida was positive against E. coli, which show that antimicrobial agents were secretory in nature. Sonicated fractions were on second order to produce antimicrobial activities. The freeze thaw method was least effective in secreting active antimicrobial agents from tested bacteria. From the present study it is concluded that microbes are natural reservoir of antimicrobial compounds.
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June 2017
Vol. 49, Iss. 3, Pages 761-1149
